Family-wise error rate

In statistics, family-wise error rate (FWER) is the probability of making one or more false discoveries, or type I errors, among all the hypotheses when performing multiple hypotheses tests.

History

Tukey first coined the term experimentwise error rate and "per-experiment" error rate for the error rate that the researcher should use as a control level in a multiple hypothesis experiment.

Background

Within the statistical framework, there are several definitions for the term "family":

• First of all, a distinction must be made between exploratory data analysis and confirmatory data analysis: for exploratory analysis – the family constitutes all inferences made and those that potentially could be made, whereas in the case of confirmatory analysis, the family must include only inferences of interest specified prior to the study.
• Hochberg & Tamhane (1987) define "family" as "any collection of inferences for which it is meaningful to take into account some combined measure of error".
• According to Cox (1982), a set of inferences should be regarded a family:

1. To take into account the selection effect due to data dredging
2. To ensure simultaneous correctness of a set of inferences as to guarantee a correct overall decision

To summarize, a family could best be defined by the potential selective inference that is being faced: A family is the smallest set of items of inference in an analysis, interchangeable about their meaning for the goal of research, from which selection of results for action, presentation or highlighting could be made (Benjamini).

Classification of multiple hypothesis tests

Main article: Classification of multiple hypothesis tests

The following table defines various errors committed when testing multiple null hypotheses. Suppose we have a number m of multiple null hypotheses, denoted by: H₁, H₂, ..., H_m. Using a statistical test, we reject the null hypothesis if the test is declared significant. We do not reject the null hypothesis if the test is non-significant. Summing the test results over H_i  will give us the following table and related random variables:

	Null hypothesis is True (H0)	Alternative hypothesis is True (H1)	Total
Declared significant	$V$	$S$	$R$
Declared non-significant	$U$	$T$	$m - R$
Total	$m_0$	$m - m_0$	$m$

• $m$ is the total number hypotheses tested
• $m_0$ is the number of true null hypotheses, an unknown parameter
• $m - m_0$ is the number of true alternative hypotheses
• $V$ is the number of false positives (Type I error) (also called "false discoveries")
• $S$ is the number of true positives (also called "true discoveries")
• $T$ is the number of false negatives (Type II error)
• $U$ is the number of true negatives
• $R$ is the number of rejected null hypotheses (also called "discoveries"). It is an observable random variable, while $S$, $T$, $U$, and $V$ are unobservable random variables.
• In $m$ hypothesis tests of which $m_0$ are true null hypotheses, $R$ is an observable random variable, and $S$, $T$, $U$, and $V$ are unobservable random variables.

Definition

The FWER is the probability of making at least one type I error in the family,

$\mathrm{FWER} = \Pr(V \ge 1), \,$

or equivalently,

$\mathrm{FWER} = 1 -\Pr(V = 0).$

Thus, by assuring $\mathrm{FWER} \le \alpha\,\! \,$, the probability of making even one type I error in the family is controlled at level $\alpha\,\!$.

A procedure controls the FWER in the weak sense if the FWER control at level $\alpha\,\!$ is guaranteed only when all null hypotheses are true (i.e. when $m_0 = m$ so the global null hypothesis is true).

A procedure controls the FWER in the strong sense if the FWER control at level $\alpha\,\!$ is guaranteed for any configuration of true and non-true null hypotheses (including the global null hypothesis).

Controlling procedures

See also: False coverage rate § Controlling procedures, and False discovery rate § Controlling procedures

Further information: List of post hoc tests

The following is a concise review of some of the classical solutions that ensure strong level $\alpha$ FWER control, followed by some newer solutions.

The Bonferroni procedure

Main article: Bonferroni correction

• Denote by $p_{i}$ the p-value for testing $H_{i}$
• reject $H_{i}$ if $p_{i} \leq \frac{\alpha}{m}$

The Šidák procedure

Main article: Šidák correction

• Testing each hypothesis at level $\alpha_{SID} = 1-(1-\alpha)^\frac{1}{m}$ is Sidak's multiple testing procedure.
• This procedure is more powerful than Bonferroni but the gain is small.
• This procedure can fail to control the FWER when the tests are negatively dependent.

Tukey's procedure

Main article: Tukey's range test

• Tukey's procedure is only applicable for pairwise comparisons.
• It assumes independence of the observations being tested, as well as equal variation across observations (homoscedasticity).
• The procedure calculates for each pair the studentized range statistic: $\frac {Y_{A}-Y_{B}} {SE}$ where $Y_{A}$ is the larger of the two means being compared, $Y_{B}$ is the smaller, and $SE$ is the standard error of the data in question.
• Tukey's test is essentially a Student's t-test, except that it corrects for family-wise error-rate.

Holm's step-down procedure (1979)

Main article: Holm–Bonferroni method

• Start by ordering the p-values (from lowest to highest) $P_{(1)} \ldots P_{(m)}$ and let the associated hypotheses be $H_{(1)} \ldots H_{(m)}$
• Let $R$ be the smallest $k$ such that $P_{(k)} > \frac{\alpha}{m+1-k}$
• Reject the null hypotheses $H_{(1)} \ldots H_{(R-1)}$. If $R = 1$ then none of the hypotheses are rejected.

This procedure is uniformly more powerful than the Bonferroni procedure. It is worth noticing here that the reason why this procedure controls the family-wise error rate for all the m hypotheses at level α in the strong sense, is because it is a closed testing procedure. As such, each intersection is tested using the simple Bonferroni test.

Hochberg's step-up procedure (1988)

Hochberg's step-up procedure (1988) is performed using the following steps:

• Start by ordering the p-values (from lowest to highest) $P_{(1)} \ldots P_{(m)}$ and let the associated hypotheses be $H_{(1)} \ldots H_{(m)}$
• For a given $\alpha$, let $R$ be the largest $k$ such that $P_{(k)} \leq \frac{\alpha}{m+1-k}$
• Reject the null hypotheses $H_{(1)} \ldots H_{(R)}$

Hochberg's procedure is more powerful than Holms'. Nevertheless, while Holm’s is a closed testing procedure (and thus, like Bonferroni, has no restriction on the joint distribution of the test statistics), Hochberg’s is based on the Simes test, so it holds only under non-negative dependence.

Dunnett's correction

Main article: Dunnett's test

Charles Dunnett (1955, 1966) described an alternative alpha error adjustment when k groups are compared to the same control group. Now known as Dunnett's test, this method is less conservative than the Bonferroni adjustment.

Scheffé's method

Main article: Scheffé's method

Resampling procedures

The procedures of Bonferroni and Holm control the FWER under any dependence structure of the p-values (or equivalently the individual test statistics). Essentially, this is achieved by accommodating a `worst-case' dependence structure (which is close to independence for most practical purposes). But such an approach is conservative if dependence is actually positive. To give an extreme example, under perfect positive dependence, there is effectively only one test and thus, the FWER is uninflated.

Accounting for the dependence structure of the p-values (or of the individual test statistics) produce more powerful procedures. This can be achieved by applying resampling methods, such as bootstrapping and permutations methods. The procedure of Westfall and Young (1993) requires a certain condition that does not always hold in practice (namely, subset pivotality). The procedures of Romano and Wolf (2005a,b) dispense with this condition and are thus more generally valid.

Alternative approaches

Further information: False discovery rate

FWER control exerts a more stringent control over false discovery compared to false discovery rate (FDR) procedures. FWER control limits the probability of at least one false discovery, whereas FDR control limits (in a loose sense) the expected proportion of false discoveries. Thus, FDR procedures have greater power at the cost of increased rates of type I errors, i.e., rejecting null hypotheses of no effect when they should be accepted.

On the other hand, FWER control is less stringent than per-family error rate control, which limits the expected number of errors per family. Because FWER control is concerned with at least one false discovery, unlike per-family error rate control it does not treat multiple simultaneous false discoveries as any worse than one false discovery. The Bonferroni correction is often considered as merely controlling the FWER, but in fact also controls the per-family error rate.

External links

• Large-scale Simultaneous Inference – Syllabus, notes, and homework from Efron's course at Stanford. Includes PDFs for each chapter of his book.