Middlebrook 7H10 Agar

Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.

Composition

• Ammonium Sulfate
• Monopotassium phosphate
• Disodium phosphate
• Sodium Citrate
• Magnesium Sulfate
• sodium
• Zinc Sulfate
• Copper Sulfate
• L-Glutamic Acid (sodium salt)
• Ferric Ammonium Citrate
• Pyridoxine Hydrochloride
• Biotin
• Malachite Green
• Agar

Cultures should be read within 5-7 days after inoculation and once a week thereafter for up to 8 weeks.

See also

• Lowenstein-Jensen medium
• Middlebrook 7H9 Broth

External links

• Middlebrook 7H10 Agar

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